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Monitoring endothelial cell dysfunction at single cell level utilising "Living Photonics" (LiPhos) concepts Monitoring endothelial cell dysfunction at single cell level utilising "Living Photonics" (LiPhos) concepts

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Date added: 03/07/2015
Date modified: 03/07/2015
Filesize: 265 Bytes
Downloads: 1573

We integrate microfluidic and label-free optical technologies with highly efficient cell-to-light coupling to measure the real-time response of individual cells in a population to specific stimuli. The innate optical response of the cell comprises of scattering, absorbance and auto-fluorescence signals as well as cell morphology. We demonstrate that the so-obtained “photonic fingerprint” acts as a key reporter for the dysfunction of cells and may thus inform on a patient’s medical condition. To perform analysis at single cell level, our centrifugal microfluidic “lab-on-a-disc” platform features a V-cup array geared for cell capture at a sharply peaked single-occupancy distribution. To support the formal process of obtaining ethical approval for access to patient samples, we demonstrate functionality on commercial human umbilical vein endothelial cells (HUVECs) and endothelial somatic cell hybrids. Differential inflammation has been induced by exposing the cells to various concentrations of protein tumour necrosis factor alpha during culture. The developed, label-free biophotonic system obtains a specific and unique photonic signal for each cell condition investigated. The level of cell dysfunction can be classified based on the obtained photonic fingerprint. The key advantage of this configuration is a highly efficient cell-to-light interaction, enabling the end user to detect the presence of diseases by measuring the cell photonic fingerprint.

Photonic profiling towards monitoring endothelial cell dysfunction at single cell level Photonic profiling towards monitoring endothelial cell dysfunction at single cell level

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Date added: 03/07/2015
Date modified: 03/07/2015
Filesize: 266 Bytes
Downloads: 1517

We integrate microfluidic and label-free optical technologies with highly efficient cell-to-light coupling to measure the real-time response of individual cells in a population to specific stimuli. The innate optical response of the cell comprises of scattering, absorbance and auto-fluorescence signals as well as cell morphology. We demonstrate that the so-obtained "photonic fingerprint" acts as a key reporter for the dysfunction of cells and may thus inform on a patient's medical condition.

Optical detection strategies for centrifugal microfluidic platforms Optical detection strategies for centrifugal microfluidic platforms

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Date added: 06/12/2014
Date modified: 06/12/2014
Filesize: 265 Bytes
Downloads: 1875

Centrifugal microfluidic systems have become one of the principal platforms for implementing bioanalytical assays, most notably for biomedical point-of-care diagnostics. These so-called ‘lab-on-a-disc’ systems primarily utilise the rotationally controlled centrifugal field in combination with capillary forces to automate a range of laboratory unit operations (LUOs) for sample preparation, such as metering, aliquoting, mixing and extraction for biofluids as well as sorting, isolation and counting of bioparticles. These centrifugal microfluidic LUOs have been regularly surveyed in the literature. However, even though absolutely essential to provide true sample-to-answer functionality of lab-on-a-disc platforms, systematic examination of associated, often optical, read-out technologies has been so far neglected. This review focusses on the history and state-of-the-art of optical read-out strategies for centrifugal microfluidic platforms, arising (commercial) application potential and future opportunities.