Details for Monitoring endothelial cell dysfunction at single cell level utilising "Living Photonics" (LiPhos) concepts

Name:Monitoring endothelial cell dysfunction at single cell level utilising "Living Photonics" (LiPhos) concepts

We integrate microfluidic and label-free optical technologies with highly efficient cell-to-light coupling to measure the real-time response of individual cells in a population to specific stimuli. The innate optical response of the cell comprises of scattering, absorbance and auto-fluorescence signals as well as cell morphology. We demonstrate that the so-obtained “photonic fingerprint” acts as a key reporter for the dysfunction of cells and may thus inform on a patient’s medical condition. To perform analysis at single cell level, our centrifugal microfluidic “lab-on-a-disc” platform features a V-cup array geared for cell capture at a sharply peaked single-occupancy distribution. To support the formal process of obtaining ethical approval for access to patient samples, we demonstrate functionality on commercial human umbilical vein endothelial cells (HUVECs) and endothelial somatic cell hybrids. Differential inflammation has been induced by exposing the cells to various concentrations of protein tumour necrosis factor alpha during culture. The developed, label-free biophotonic system obtains a specific and unique photonic signal for each cell condition investigated. The level of cell dysfunction can be classified based on the obtained photonic fingerprint. The key advantage of this configuration is a highly efficient cell-to-light interaction, enabling the end user to detect the presence of diseases by measuring the cell photonic fingerprint.

Authors:King, D., Macdara, G., Burger, R., Kurzbuch, D., Kearney, S., Ducrée, J.
Journal Title:Proc. 4th European Conference on Microfluidics
Journal Year:2014
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Created On: 03/07/2015 16:38
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